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Introduction: Extracted human teeth have been used as a common mode to train undergraduate dental students before they step into the clinical year and start practicing on live patients. The sources of infections such as saliva, blood, and body fluids that are present in clinical settings may contaminate and exist in the extracted stored teeth which are used further for preclinical education.

Objective: To assess the attitude /knowledge of handling of extracted teeth among the dental students of Kathmandu Medical and Dental College, Kathmandu, Nepal.

Materials and Method: This is a cross-sectional, descriptive study. A formulated closed-ended questionnaire was used for collecting the data. All the data obtained were entered and tabulated in MS- Excel, and further descriptive calculations like average and total were done using MS–Excel. 

Result: Altogether 180 students participated in the study. The use of extracted teeth was found to be highest in Conservative Dentistry and Endodontics. Students collected the extracted teeth from the private clinic in a more number compared to the institute and the quacks. Most of the students used sodium hypochlorite for sterilizing the extracted teeth before starting the procedure. Students used general garbage bins for discarding the used extracted teeth.

Conclusion: The knowledge, performance, and attitude of dental students in relation to sterilization or disinfection methods of extracted human teeth were quite satisfactory and students in this study displayed a positive level of knowledge regarding sterilization and infection control practices.

Dental education; disinfection; extracted teeth.