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Introduction: Besides proper biomechanical preparation and obturation, microleakage of sealing materials is an important parameter that determines the success of endodontic treatment.

Objective: To compare the sealing ability of MTA-based root canal sealer, MTA Fillapex with resin-based AH Plus and silicone-based GuttaFlow.

Materials and Method: Ninety-five non-carious, single-rooted mandibular premolars were collected and immersed in sodium hypochlorite (5.25%, 24 hours). Selected teeth were decoronated to obtain 13 mm length. After decoronation, root canals of all teeth were prepared by Protaper with an apical preparation up to size F-3. The irrigant used was 5.25% sodium hypochlorite. After cleaning and shaping, samples were divided into three experimental groups of 30 each and five samples in positive control group. The experimental groups were obturated with MTA Fillapex, GuttaFlow, and AH Plus whereas fourth group served as control group which was instrumented but not obturated. Coronal cavities were then filled with glassionomer cement (3 mm). All samples were coated with two layers of nail varnish except 5 mm apically, immersed in 5% methylene blue dye for 48 hours, then washed under tap water and nail varnish removed. The samples were sectioned longitudinally and extent of apical dye penetration in all groups were measured.

Result: MTA Fillapex had minimum linear microleakage compared to both GuttaFlow and AH Plus but the difference was not significant with GuttaFlow. Whereas, AH Plus showed significantly more microleakage.

Conclusion: MTA Fillapex exhibited better sealing ability than GuttaFlow and AH Plus, though the mean leakage scores were statistically insignificant with GuttaFlow.

Dye penetration; methylene blue; microleakage; mineral trioxide aggregate; resin-based sealer; silicone-based sealer.