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Introduction: The objective of root canal therapy is the extermination of noxious microorganisms from root canal space. Thus, for the accomplishment of successful root canal therapy, cleaning and shaping, and obturation are important parameters.

Objective: To assess and compare sealer distribution in root canal using two different sealers in obturated root canals.

Materials and Method: This in vitro study was conducted in the Department of Conservative Dentistry and Endodontics of the medical institute from July to August 2020 after obtaining ethical clearance. Twenty freshly extracted single-rooted anterior teeth obtained by convenience sampling method were included. All the specimens were divided into two study groups with 10 specimens in each group. One group consisted of specimen in which AH plus sealer was used while in the other group specimens, Tubliseal EWT sealer was used. Mixing of all the sealers was done following manufacturer’s instructions. Obturation was done in all the specimens. After completion of obturation, storing of the specimens was done for seven days. This was followed by horizontal sectioning at 3 mm from the apex. Stereo microscopic examination of the specimens was done and percentage of sealer coated perimeter (PSCP) of canal was done.

Result: Mean PSCP of the AH plus sealer group was 96.65 mm while mean PSCP of Tubliseal EWT group was 97.41 mm. While comparing the mean PSCP among the two study groups, non-significant results were obtained.

Conclusion: Both AH plus and Tubliseal EWT can be used with equal efficacy in patients undergoing root canal therapy.

In vitro; root canal therapy; sealer.